Hi guys,
I know that the HI-Q seq enzyme is available for the PGM...any impressions?
Remito
I know that the HI-Q seq enzyme is available for the PGM...any impressions?
Remito
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According to sales reps, you must buy if you want longer - 400bp reads -
I've run lots of runs with both the old 400 bp sequencing kit and then now the new Hi-Q kit. In my hands, the Hi-Q kit seems to outperform the old kits. I haven't found a downside yet. I gather the only thing they have changed is a new sequencing polymerase and a modification to the Wash 2 buffer. This has resulted in higher key signal for us and better quality scores in the longer reads, i.e. less 3' trimming. It's also essentially the same price, maybe $25 more per run, so I think the cost is negligible.Comment
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