barcode library for ion torrent

[linda2015]

Hi everybody, I am new in the NGS field. I want to ask a question. If I want to barcode my samples, should I do paired barcode or one side barcode, considering the reading length of ion torrent, If the fragment is more than 300bp, maybe there will be an issue to do paired barcod?

I want to do a pilot experiment to test my propose. but I hardly find literature regarding to my question.

I wonder if any of you have experience on this, can you share your thoughts?

Greatly appreciated!

[maven]

One barcode because Ion Torrent software doesn't look for barcodes on the other ends.

[linda2015]

One barcode because Ion Torrent software doesn't look for barcodes on the other ends.

thanks for your reply,
Another question is, I am not sure in which end to put barcode, in the beads end, is it P1 adapter and the other is A adpater? If I want to add barcode for sucessful read through, which end is better.

Thanks a lot