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SNP detection in Draft genomes

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SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
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  • SNP detection in Draft genomes

    Hi all,
    We have sequenced a Bacteria on Ion PGM platform for Denovo sequencing. We did get a good scaffold data.

    Our next aim is to take this as a reference sequence and compare it with remaining samples which were also sequenced on IonPGM.

    Now do i have to order the contigs or scaffolds and then go for SNP search?
    What are the tools which can be used for SNP predictions from draft genomes?
    How do we annotate these SNP's?

    Thanks in advance,

    Ramya

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