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  • zcm2403
    Junior Member
    • Dec 2014
    • 9

    #1

    PGM CHIP loading problem

    This is the chip i have loaded today.I'm confused why the loading is so bad.As you see in the image,the upper half is so dense,the lower half is so sparse.can anyone tell me the cause?Thanks!
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  • r.rosati
    Member
    • Aug 2015
    • 95

    #2
    Newbie here, and I actually use an Ion Proton - but how likely is it that the chip wasn't dry when you loaded the ISPs? It looks like the sample entered the chip by displacing another solution - in this case the two blue "vortexes" and the bottom part of the chip would be where the solution lingered.
    Alternatively... a warped chip, perhaps?
    Last edited by r.rosati; 08-05-2015, 03:44 PM.

    Comment

    • zcm2403
      Junior Member
      • Dec 2014
      • 9

      #3
      Originally posted by r.rosati View Post
      Newbie here, and I actually use an Ion Proton - but how likely is it that the chip wasn't dry when you loaded the ISPs? It looks like the sample entered the chip by displacing another solution - in this case the two blue "vortexes" and the bottom part of the chip would be where the solution lingered.
      Alternatively... a warped chip, perhaps?

      Thanks for your answer!I'll try your advice next time!

      Comment

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