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  • molpath
    Junior Member
    • Aug 2015
    • 1
    #1

    Low final library

    Hi everyone! I am brand new Ion Torrent user, and I tried to prepare a run for inherited disease panel. In the end, I got a very poor Final Library result,only 3% in which I see that most of the unusable reads were test fragments, 82%.Here is the screenshot.
    [IMG]/Users/Riko/Desktop/5b37c3eba942a8df384012e4c200dd8d0b4a601f70d2e2296f707f1a11d8a234.0.jpg[/IMG]

    Can You please tell me possible reasons for this?
    Tags: None
    • jon123
      Junior Member
      • Jun 2015
      • 4
      #2
      Could you please tell me your i)amount and size distribution of input DNA for library preparation, ii)final library images by bioanalyzer, and iii)library kit.

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