Hi all, I'm preparing to do a new sequencing run on the Ion Torrent PGM. I use the NebNext Fast DNA Fragmentation & Library Prep kit to prepare my library. I, as most, would like to be as cost efficient as possible and wondered if anyone had tried to reduce the amount of reagents used per reaction by half so that I could prep 2 samples as opposed to just one with the same amount of kit components. Thoughts?
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...Today, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...