Hi all, I'm preparing to do a new sequencing run on the Ion Torrent PGM. I use the NebNext Fast DNA Fragmentation & Library Prep kit to prepare my library. I, as most, would like to be as cost efficient as possible and wondered if anyone had tried to reduce the amount of reagents used per reaction by half so that I could prep 2 samples as opposed to just one with the same amount of kit components. Thoughts?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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