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  • priyanka_15
    Member
    • May 2015
    • 17
    #1

    low library yield with Ion AmpliSeq Inherited disease panel

    Hi All

    I am facing very low yield in library when using 10ng gDNA per pool for Inherited disease panel. I have tried many things like increasing DNA amount, target amplification cycles, ensuring fresh 70% ethanol, drying the beads ( I do 2-3 min. as the place we live is very dry). According to protocol, I should get 300ng/ml-1500ng/ml but I am only getting 132-182ng/ml.

    Any ideas or advice?

    Thanks
    Tags: ion torrent, library amplification, low yield
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