Good evening people,
I am working in a lab where we use S5 IonTorrent sequencer and we are sequencing with Colon and Lung V2 panel different type of samples: colon cancers, lung cancers, ovarian cancer, liver cancer, also we sequenced few samples from buccal swab for experiment. In 80% plus samples sequenced with this NGS panel we detect 4 repeating mutations: FGFR3:1807894 G>A; TP53:7579472 G>C; EGFR:55228053 A>T; EGFR:55249063 G>A. At first we thought it was contamination then we repeat same samples with re isolated DNA and we got same result, also we changed library preparation kit, we also asked for colleagues who used same panel do they get same mutations and they do. After 2 years of sequencing we conclude it is not contamination but something different. Could you, please, give me some information about this issue we have?
Sincerely AGC team.
I am working in a lab where we use S5 IonTorrent sequencer and we are sequencing with Colon and Lung V2 panel different type of samples: colon cancers, lung cancers, ovarian cancer, liver cancer, also we sequenced few samples from buccal swab for experiment. In 80% plus samples sequenced with this NGS panel we detect 4 repeating mutations: FGFR3:1807894 G>A; TP53:7579472 G>C; EGFR:55228053 A>T; EGFR:55249063 G>A. At first we thought it was contamination then we repeat same samples with re isolated DNA and we got same result, also we changed library preparation kit, we also asked for colleagues who used same panel do they get same mutations and they do. After 2 years of sequencing we conclude it is not contamination but something different. Could you, please, give me some information about this issue we have?
Sincerely AGC team.
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