you probably accidentally lost your sample in one of the cleanup steps.

[lsmiles]

I have a question about the protocol... I was using the Ion Xpress Library kit and was getting no concentration after the amplification step (sample was in water, so no EDTA was present). I had plenty before this step in the region for the size select, so I am not sure why the concentration goes down to nothing. The Ion Xpress Plus Library Prep kit is on back-order... I have heard that this will resolve the issue of no concentration, but what killed my concentration in the first place?

[krobison]

But how do you realize if you have a good shot at winning, if for instance, you don't have access to the protocols etc! 75K is a tough ticket if you want to have a stab!

,:0)

YUP! I've moaned about this repeatedly -- I strongly believe Ion is hurting themselves by their various levels of hiding information from prospective customers and collaborators, but they've never budged.

[DICER]

Well, they have through the website. To actually enter any of the challenges involving experiments (not the pure software one), you do actually have to own a PGM. Obviously, if you can get to the protocols, you could try experimenting with the protocol without having a PGM (or borrowing access to a friend's).

Of course, if you thought you had a good shot at $1M, you'd invest $75K to win it!

But how do you realize if you have a good shot at winning, if for instance, you don't have access to the protocols etc! 75K is a tough ticket if you want to have a stab!

,:0)

[DICER]

Loving the lively discussion and input!

To clarify, all current PGM users, developers and Grand Challenge participants are able to access through the IonCommunity, every protocol, user guide, data set, and application note that we have released. The amount of user-generated content and user-inspired discussion threads on the Community also continues to grow. We really encourage users to check it out.

If you would like more details about the technology and our workflow, but don't fit the above bill, there are still several options for you to learn more about Ion:

1) Register for the IonCommunity, which provides a deeper level of content (e.g., videos, info on the Grand Challenges, and upcoming events and webinars) than what is available on our website

2) Contact your local Life Technology/Ion Torrent representative who will be more than happy to speak with you and find a way to get you the information/data you need

We've been scratching at being more open to the broader community (i.e., making the TMAP aligner source code available, disclosing our entire 2011 product roadmap with 316/318/OneTouch, etc.). Clearly, we can do better by all of you, and welcome the swift kick in the a$$ to get us there even faster.

-The Ion Torrent Team

Releasing Paired End Protocols and adaptor sequences would be not only stimulating and useful for the Ion community, it would encourage transparency and promote robust protocol development, for PhD students, post-docs and buyers alike. A grand challenge!

,:0)

[krobison]

That limits the potential participants of the "grand challenge" to PGM users if you have no idea the protocols, data, etc. that you are working to improve. They need to post the baseline.

Well, they have through the website. To actually enter any of the challenges involving experiments (not the pure software one), you do actually have to own a PGM. Obviously, if you can get to the protocols, you could try experimenting with the protocol without having a PGM (or borrowing access to a friend's).

Of course, if you thought you had a good shot at $1M, you'd invest $75K to win it!

[IonTorrent]

Loving the lively discussion and input!

To clarify, all current PGM users, developers and Grand Challenge participants are able to access through the IonCommunity, every protocol, user guide, data set, and application note that we have released. The amount of user-generated content and user-inspired discussion threads on the Community also continues to grow. We really encourage users to check it out.

If you would like more details about the technology and our workflow, but don't fit the above bill, there are still several options for you to learn more about Ion:

1) Register for the IonCommunity, which provides a deeper level of content (e.g., videos, info on the Grand Challenges, and upcoming events and webinars) than what is available on our website

2) Contact your local Life Technology/Ion Torrent representative who will be more than happy to speak with you and find a way to get you the information/data you need

We've been scratching at being more open to the broader community (i.e., making the TMAP aligner source code available, disclosing our entire 2011 product roadmap with 316/318/OneTouch, etc.). Clearly, we can do better by all of you, and welcome the swift kick in the a$$ to get us there even faster.

-The Ion Torrent Team

[epistatic]

That limits the potential participants of the "grand challenge" to PGM users if you have no idea the protocols, data, etc. that you are working to improve. They need to post the baseline.

[GW_OK]

Like some big biotech couldn't get the protocols through back channels. What a jerk move.

[NextGenSeq]

Wow, that was fast.

I guess they are concerned about other vendors making kits for their system.

[ECO]

NextGenSeq, I just received the following private message (and several emails) from Ion Torrent, so I had to take down the user protocols you just posted, sincerest apologies to you and anyone else who might be interested in them.

Dear Eric,

We request that the following post be removed:

<url removed>

Ion Torrent is still in a rapid growth phase, and we expect our protocols to be continued to be enhanced in the coming weeks/months. We want to avoid confusion in the market place by having this exist as a permanent link on an external website.

Secondarily, posting of protocols on other websites is a direct violation of our terms and conditions.

Thank you for your help,
Ion Torrent

[estibalizolano]

same here ...

I also would love to have the ionTorrent emPCR - moslty to compare methods and get an idea of what it really involves, equipment needed ... Anyway, I leave my e-mail address here in case someone can help with the protocol? [email protected]
THANKS

[ECO]

Ion Community Home>Polls>Useful information release
Voting starts on May 9, 2011 12:00 AM

What would be the most useful information to be posted on the Ion Community?
Vote now: choose one

1. Simplicity! Releasing the protocols for the kits will show how simple the entire process is.
2. Science! Releasing the protocols for the kits will help develop more scientific applications of the PGM.
3. Speed! Releasing the protocols for the kits will show how quickly I can get from sample to data.
4. Data produced by the PGM! Showing how well the PGM sequences will support purchases.
5. Nothing! Keeping information behind closed doors doesn't build a community

.

Admirable next step, pretty funny that there is no option for ALL.

[epistatic]

Ion Community Home>Polls>Useful information release
Voting starts on May 9, 2011 12:00 AM

What would be the most useful information to be posted on the Ion Community?
Vote now: choose one

1. Simplicity! Releasing the protocols for the kits will show how simple the entire process is.
2. Science! Releasing the protocols for the kits will help develop more scientific applications of the PGM.
3. Speed! Releasing the protocols for the kits will show how quickly I can get from sample to data.
4. Data produced by the PGM! Showing how well the PGM sequences will support purchases.
5. Nothing! Keeping information behind closed doors doesn't build a community

.

[GW_OK]

You know I actually talked with Jonathan Rothberg at AGBT 2010, when the ion torrent was first being announced and unless I am misremembering the whole ethos of the company was going to be tinkerer-friendly towards reagents and code, with the major focus of the company being set on the selling of the chips. I can understand Life Tech (as formerly ABI) being all about the proprietarization (is that a word?) but you know if you really want the envelope pushed with your tech you need to let people start messing with it.