Hello everyone,
I am having problems with ampliseq library preparation. I don't get high yield of library prep. I suspect that I vortexed too much in each step. I was told to vortex all the enzymes (HIFI, Fupu, ligase) before used and vortex after adding the enzymes to reactions to mix. I am not sure if we should vortex the enzymes and if we should vortex for each step. I am afraid enzymes will be damaged and DNA will be sheared by vortexing. I wonder if anyone has problems by vortexing or if it is ok to vortex.
Thank you.
I am having problems with ampliseq library preparation. I don't get high yield of library prep. I suspect that I vortexed too much in each step. I was told to vortex all the enzymes (HIFI, Fupu, ligase) before used and vortex after adding the enzymes to reactions to mix. I am not sure if we should vortex the enzymes and if we should vortex for each step. I am afraid enzymes will be damaged and DNA will be sheared by vortexing. I wonder if anyone has problems by vortexing or if it is ok to vortex.
Thank you.
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