Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Jackie Jalie
    replied
    The Ampliseq program automatically performs a number of preprocessing steps on your sequence data, including quality control, trimming and normalization. The preprocessing stage also includes an alignment algorithm that attempts to identify homologous regions in the sequences, with the goal of producing an aligned capillary dataset that can be used for downstream analyses such as cDNA synthesis. I will have to get online mba assignment help so that I could do other things with my friends who is having wedding next week.

    Leave a comment:


  • Sunny88
    started a topic Vortex during Ampliseq library preparation

    Vortex during Ampliseq library preparation

    Hello everyone,

    I am having problems with ampliseq library preparation. I don't get high yield of library prep. I suspect that I vortexed too much in each step. I was told to vortex all the enzymes (HIFI, Fupu, ligase) before used and vortex after adding the enzymes to reactions to mix. I am not sure if we should vortex the enzymes and if we should vortex for each step. I am afraid enzymes will be damaged and DNA will be sheared by vortexing. I wonder if anyone has problems by vortexing or if it is ok to vortex.

    Thank you.

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 07:49 AM
0 responses
4 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 07:23 AM
0 responses
9 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-17-2024, 06:54 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-14-2024, 07:24 AM
0 responses
24 views
0 likes
Last Post seqadmin  
Working...
X