Argon

[jcosta]

Hi all,

Can any of the Ion Torrent user let me know the Argon consumption levels of their machines? I will soon get the machine and just today was told that it requires argon as a carrier
Thanks,

[Claudia Stewart]

We have had our PGM since the end of Frebruary, made 48 runs and still have not needed to replace. We were told that we could expect to replace the tank every 4-6 weeks if we ran it every day.

[domski]

argon tank

Hello Claudia,

How big is your argon tank, in cubic feet (CF)? Thanks.

Alan

We have had our PGM since the end of Frebruary, made 48 runs and still have not needed to replace. We were told that we could expect to replace the tank every 4-6 weeks if we ran it every day.

[Hiro.Protagonist]

40 CF lasts about a month.

Wash out the used chips and run again. Put into centrifuge and spin upside down to get things out of the well. Just don't let them dry out.

[n2onme]

40 CF lasts about a month.

Wash out the used chips and run again. Put into centrifuge and spin upside down to get things out of the well. Just don't let them dry out.

Do you have any data after washing out the chip? I was going to try this as well.

[diagen]

Thank you. That is very nice idea.

And now next question:
What you do with used dNTP?

SAP phosphatase can degrade dNTPs.

[Gorbenzer]

i would like to know too how you wash your used chips! If we could use them again would be really nice!

Pls give a short explanation!!

I'll start a topic!

http://seqanswers.com/forums/showthread.php?t=20695

[diagen]

Use sonication bath prior centrifuging?