Hello,
My lab is currently having trouble getting 70-80% template positive ISP for our Post-Enrichment product.(Our data is MUCH lower)
It is almost a random occurrence when some emulsions will provide excellent results and some are very complete failures.
We are wondering if anyone can attribute these hicupps to certain steps in the protocol or if anyone has found a particular reason for this anomaly. I would like to find a way to test our samples before running them in the sequencer to determine if our run will be a success or a failure.
Any ideas or thoughts are welcome
-MDnextgenseq
My lab is currently having trouble getting 70-80% template positive ISP for our Post-Enrichment product.(Our data is MUCH lower)
It is almost a random occurrence when some emulsions will provide excellent results and some are very complete failures.
We are wondering if anyone can attribute these hicupps to certain steps in the protocol or if anyone has found a particular reason for this anomaly. I would like to find a way to test our samples before running them in the sequencer to determine if our run will be a success or a failure.
Any ideas or thoughts are welcome
-MDnextgenseq
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