Hi everyone,
Recently I just received PEreads from BGI-seq, and the sequencer company provide me with this adapters index:
.......etc.....
So, I used those shrt sequences to trim my reads using trimmomatic in Galaxy, and prepared the fasta seq as follow for trimming:
>index1/1
GGTCCATT
>index1/2
TTAGGTAG
>index2/1
GGTCCATT
>index2/2
GGTCACTA
>index3/1
GGTCCATT
>index3/2
TCCGCACT
...etc...
Did I use the information correctly? because recently I just found that others have been using:
forward_adapter = "AAGTCGGAGGCCAAGCGGTCTTAGGAAGACAA"
reverse_adapter = "AAGTCGGATCGTAGCCATGTCGTTCTGTGAGCCAAGGAGTTG"
to trim the adapters, not sure if its for SE only or also PE. Thank you
Recently I just received PEreads from BGI-seq, and the sequencer company provide me with this adapters index:
i7_Index_ID | i7index配列 | i5_Index_ID | i5index配列 | ||
DNA | Index | i7ID | i7Seq | i5ID | i5Seq |
SG-003 | M5041/M7091 | M7091 | CAATCGTT | M5041 | CTGCGGAT |
SG-015 | M5041/M7092 | M7092 | AGCCATAC | M5041 | CTGCGGAT |
SG-37 | M5041/M7093 | M7093 | GTGATAGG | M5041 | CTGCGGAT |
SG-55 | M5041/M7094 | M7094 | TCTGGCCA | M5041 | CTGCGGAT |
SG-77 | M5041/M7101 | M7101 | TATGAACA | M5041 | CTGCGGAT |
So, I used those shrt sequences to trim my reads using trimmomatic in Galaxy, and prepared the fasta seq as follow for trimming:
>index1/1
GGTCCATT
>index1/2
TTAGGTAG
>index2/1
GGTCCATT
>index2/2
GGTCACTA
>index3/1
GGTCCATT
>index3/2
TCCGCACT
...etc...
Did I use the information correctly? because recently I just found that others have been using:
forward_adapter = "AAGTCGGAGGCCAAGCGGTCTTAGGAAGACAA"
reverse_adapter = "AAGTCGGATCGTAGCCATGTCGTTCTGTGAGCCAAGGAGTTG"
to trim the adapters, not sure if its for SE only or also PE. Thank you