Originally posted by ymc
View Post
-
Matching the error rate of PacBio would indeed be pretty impressive, but that alone is insufficient to kill off PacBio or Illumina. The question is does the ONT error profile match that of PacBio? In other words, is it stochastic and relatively free of GC bias so that at sufficient coverage the consensus error rate drops to very low levels (like PacBio). Or does it suffer from systematic errors that create a ceiling to the consensus read quality? Have these questions been definitely answered yet? -
If the accuracy of R9 chemistry plus RNN algorithm is true, then both PacBio and Illumina will be dead.
However, judging from ONT's past record, this might well be achievable but the timeline is more likely to be one or two years in the future. As a result, this lawsuit can still be a distraction for ONT for the time being IMHO.Leave a comment:
-
A Hammer Thrown at Big Brother?
Oxford Nanopore Technologies, the company that pioneered a new DNA analysis technique called nanopore sequencing, has remained secretive about the microscopic channel at the heart of its products. But on the heels of a patent infringement lawsuit from sequencing behemoth Illumina, Inc., the company has revealed what will drive its newest devices—a bacterial pore that seems to circumvent Illumina’s challenge.
...
A webcast presentation today for customers of the company will likely put many of those fears to rest. Oxford Nanopore’s Chief Technology Officer Clive Brown announced an upgrade to its devices—a new pore the company has been describing in presentations as R9. Brown revealed that R9 is CsgG, a membrane protein derived from Escherichia coli, but present in many bacterial species.
http://www.sciencemag.org/news/2016/...new-technology
-
Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.
Methylation Detect...Yesterday, 10:46 AM -
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...05-19-2023, 10:03 AM
Leave a comment: