Tweet
Has anyone tried using a different TA ligase for library preparation? We've got a ton of KAPA Hyper Prep reagents (KK8503) which as far as I can work out can like for like substitute for the NEB Blunt/TA ligase master mix (and the NEB End repair/dA-tailing master mix though this isn't needed in the rapid library preparation). It seems a waste of cash to buy in new enzymes which we already have by the bucket load!
-
-
Yes, I ran a flow cell in someone else's lab using some random other ligase that they had lying round. They just mixed the ligase with a buffer in the required concentration, and it was loaded as normal. The run worked, although I didn't have any comparable TA/ligase runs to benchmark against.
With the tiny amount required, you probably don't need the buffer, and could possibly get away with just adding a little bit more of anything that is ligase-like.
-
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM
Comment