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Barcode contamination in nanopore



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  • Barcode contamination in nanopore

    We have used 24 barcodes in 1 flow cell for a 16s run using SQK-16S024.The flow cell has been washed. Now we want to run more samples for a 16s run using the same barcodes in the kit on the same flowcell. We have heard this causes cross contamination in the new run due to leftover previous template.
    Can somebody let me know the % of contamination expected from previous run?
    People have suggested to use different set of barcodes in nanopore as a solution to this, but practically this is not possible since we dont want to buy multiple barcode kits and many flowcells.
    Have somebody ever had this experience before?

  • #2
    Based on Nanopore's own research, the WSH004 kit has less than 1% carry over: https://community.nanoporetech.com/p...ash-kit-exp-ws.


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