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  • cascoamarillo
    started a topic HGAP assembly failed task on SMRT Portal

    HGAP assembly failed task on SMRT Portal

    Hi

    So I have 10 SMRT cells that I've been playing with. But de novo assembly with protocols RS_HGAP_Assembly.2 and .3 on SMRT Portal shows an error message during the process. Using smrtanalysis_2.3.0.140936.run with smrtanalysis-patch_2.3.0.140936.p3.run.

    Code:
    [INFO] 2015-04-28 22:02:21,943 [smrtpipe.status refreshTargets 409] Workflow Completion Status 139/212 in ( ...... 65%) tasks completed.
    [ERROR] 2015-04-29 09:52:35,198 [smrtpipe.status refreshTargets 413] *** Failed task task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_006of006
    [ERROR] 2015-04-29 09:52:35,199 [smrtpipe.status refreshTargets 413] *** Failed task task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_004of006
    [ERROR] 2015-04-29 09:52:35,199 [smrtpipe.status refreshTargets 413] *** Failed task task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_005of006
    [ERROR] 2015-04-29 09:52:35,199 [smrtpipe.status refreshTargets 413] *** Failed task task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_003of006
    [ERROR] 2015-04-29 09:52:35,200 [smrtpipe.status refreshTargets 413] *** Failed task task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_001of006
    [ERROR] 2015-04-29 09:52:35,200 [smrtpipe.status refreshTargets 413] *** Failed task task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_002of006
    [INFO] 2015-04-29 09:52:35,212 [smrtpipe.status execute 627] Found 6 failed tasks.
    [INFO] 2015-04-29 09:52:35,213 [smrtpipe.status execute 629] task hgapAlignForCorrection_004of006 FAILED
    [INFO] 2015-04-29 09:52:35,213 [smrtpipe.status execute 629] task hgapAlignForCorrection_003of006 FAILED
    [INFO] 2015-04-29 09:52:35,214 [smrtpipe.status execute 629] task hgapAlignForCorrection_006of006 FAILED
    [INFO] 2015-04-29 09:52:35,214 [smrtpipe.status execute 629] task hgapAlignForCorrection_001of006 FAILED
    [INFO] 2015-04-29 09:52:35,215 [smrtpipe.status execute 629] task hgapAlignForCorrection_002of006 FAILED
    [INFO] 2015-04-29 09:52:35,215 [smrtpipe.status execute 629] task hgapAlignForCorrection_005of006 FAILED
    [ERROR] 2015-04-29 09:53:06,302 [SMRTpipe.SmrtPipeMain run 608] SmrtExit task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_004of006 Failed task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_003of006 Failed task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_006of006 Failed task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_001of006 Failed task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_002of006 Failed task://016450/P_PreAssemblerDagcon/hgapAlignForCorrection_005of006 Failed
    that's part of log file where the errors start showing up. Not sure where is the problem.
    Could it be due to a genome size limitation? Working with eukaryote genome (ca. 200Mb)
    Thanks

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