I want to do methylation analysis based on pacbio .bax.h5 data. I already mapped the .h5 data to the reference genome and got .cmp.h5/.sam results.
In order to get IPD information, I knew from the pacbio website that kinetics tools can be used for extracting IPD from .cmp.h5. But I couldn't find detailed documentations of how to install/use the kinetics tools.
Has anyone ever used such tools to get IPD information and corresponding analysis of methylation? Or are there any other methods for these analysis?
Thanks a lot
In order to get IPD information, I knew from the pacbio website that kinetics tools can be used for extracting IPD from .cmp.h5. But I couldn't find detailed documentations of how to install/use the kinetics tools.
Has anyone ever used such tools to get IPD information and corresponding analysis of methylation? Or are there any other methods for these analysis?
Thanks a lot
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