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  • letunglam
    Junior Member
    • Jan 2018
    • 3

    #1

    Import raw data to SMRTLink

    I find raw data (bam file, subread.xml..) from Pacbio. I added to SMRTLink. but when I use this data to assembly by HAGP4, SMRTLink noticed fail.
    Can I help me how to add raw data to SMRTLink?
    P/s: We have a Pacbio SQUEL.

    This is log file when i add subread file to SMRTLink.
    [INFO] [2018-01-29T21:37:05.659-05:00] Running job-type import_dataset com.pacbio.secondary.analysis.jobtypes.ImportDataSetJob@42d380d9 in /opt/pacbio/smrtlink/userdata/jobs_root/000/000037
    [INFO] [2018-01-29T21:37:06.440-05:00] Can't process this dataset
    [INFO] [2018-01-29T21:37:06.485-05:00] Completed running job import_dataset Result -> Success(Right(PacBioDataStore(2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,0.2.1,List(DataStoreFile(36c19ac0-c34b-fc6b-86f2-743a3da771e3,pbscala::import_dataset,PacBio.DataSet.SubreadSet,2410,2018-01-29T21:37:05.659-05:00,2018-01-29T21:37:05.659-05:00,/home/data/pacbio/raw_data/Arabidopsis_data_demo/3_C01_customer/m54113_160914_092411.subreadset.xml,false,pacbio_dataset_subreadset-160923_085639252,Imported DataSet on 2018-01-29T21:37:05.659-05:00), DataStoreFile(94faee66-ef35-4d00-9d05-ea98db2eafa3,pbsmrtpipe::master.log,PacBio.FileTypes.log,0,2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,/opt/pacbio/smrtlink/userdata/jobs_root/000/000037/pbscala-job.stdout,false,Job Master Log,Job Master log of the Import Dataset job), DataStoreFile(8f017810-09fb-481a-9cde-a702fabaa597,pbscala::dataset_report,PacBio.FileTypes.JsonReport,397,2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,/opt/pacbio/smrtlink/userdata/jobs_root/000/000037/dataset-reports/simple_dataset_report.json,false,PacBio Report simple_dataset_report,PacBio DataSet Report for simple_dataset_report)))))
    [INFO] [2018-01-29T21:37:06.485-05:00] attempting to import
    PacBioDataStore Summary 3 files Created at 2018-01-29T21:37:06.485-05:00 Schema version 0.2.1
    1. DataStoreFile(36c19ac0-c34b-fc6b-86f2-743a3da771e3,pbscala::import_dataset,PacBio.DataSet.SubreadSet,2410,2018-01-29T21:37:05.659-05:00,2018-01-29T21:37:05.659-05:00,/home/data/pacbio/raw_data/Arabidopsis_data_demo/3_C01_customer/m54113_160914_092411.subreadset.xml,false,pacbio_dataset_subreadset-160923_085639252,Imported DataSet on 2018-01-29T21:37:05.659-05:00)
    2. DataStoreFile(94faee66-ef35-4d00-9d05-ea98db2eafa3,pbsmrtpipe::master.log,PacBio.FileTypes.log,0,2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,/opt/pacbio/smrtlink/userdata/jobs_root/000/000037/pbscala-job.stdout,false,Job Master Log,Job Master log of the Import Dataset job)
    3. DataStoreFile(8f017810-09fb-481a-9cde-a702fabaa597,pbscala::dataset_report,PacBio.FileTypes.JsonReport,397,2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,/opt/pacbio/smrtlink/userdata/jobs_root/000/000037/dataset-reports/simple_dataset_report.json,false,PacBio Report simple_dataset_report,PacBio DataSet Report for simple_dataset_report)
    [INFO] [2018-01-29T21:37:06.568-05:00] Successfully inserted datastore files:
    [INFO] [2018-01-29T21:37:06.568-05:00] Successfully entered SubreadSet SubreadServiceDataSet(-99,36c19ac0-c34b-fc6b-86f2-743a3da771e3,pacbio_dataset_subreadset-160923_085639252,/home/data/pacbio/raw_data/Arabidopsis_data_demo/3_C01_customer/m54113_160914_092411.subreadset.xml,2018-01-29T21:37:06.544-05:00,2018-01-29T21:37:06.544-05:00,573889,5347463396,3.0.1, ,subreadset,9a6026d88580d6130acbbe2d28e98c55,54113,m54113_160914_092411,PacBio_LER0_32kbLibrary_1_3_C01,3_C01,unknown,0,PacBio_LER0_32kbLibrary_1_3_C01,None,37,1,PacBio.DataSet.SubreadSet)
    Unsupported DataSet type PacBio.FileTypes.log. Imported DataStoreFile(94faee66-ef35-4d00-9d05-ea98db2eafa3,pbsmrtpipe::master.log,PacBio.FileTypes.log,0,2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,/opt/pacbio/smrtlink/userdata/jobs_root/000/000037/pbscala-job.stdout,false,Job Master Log,Job Master log of the Import Dataset job). Skipping extended/detailed importing
    Unsupported DataSet type PacBio.FileTypes.JsonReport. Imported DataStoreFile(8f017810-09fb-481a-9cde-a702fabaa597,pbscala::dataset_report,PacBio.FileTypes.JsonReport,397,2018-01-29T21:37:06.485-05:00,2018-01-29T21:37:06.485-05:00,/opt/pacbio/smrtlink/userdata/jobs_root/000/000037/dataset-reports/simple_dataset_report.json,false,PacBio Report simple_dataset_report,PacBio DataSet Report for simple_dataset_report). Skipping extended/detailed importing
  • rhall
    Senior Member
    • Aug 2012
    • 324

    #2
    For a quick resolution, file a ticket with Pacbio tech support.

    Alternatively, what version of SMRT Link are you using, and where did the data come from?

    Comment

    • letunglam
      Junior Member
      • Jan 2018
      • 3

      #3
      Originally posted by rhall View Post
      For a quick resolution, file a ticket with Pacbio tech support.

      Alternatively, what version of SMRT Link are you using, and where did the data come from?
      SMRT Link version is 5.01
      I get data from pacbio: https://www.pacb.com/blog/sequel-sys...nome-assembly/

      Comment

      • rhall
        Senior Member
        • Aug 2012
        • 324

        #4
        Can you try on the command line
        Code:
        pbservice import-dataset --host <hostname> --port <port> m54113_160914_092411.subreadset.xml --verbose

        Comment

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