Trimming reads and reanalyzing using Seq Scanner

[tj11]

Hello - After the run is sequenced I would like to trim reads and re-analyze the reads using Seq Scanner v1.0. How do I go about it?

[westerman]

You are going to need to be more specific in your question. Such as the exact step in which you are having problems.

[tj11]

I did a run and viewed the sequence through the sequence scanner v1. However I would like to edit (trim the read) so I can re-analyze it using the software. How do I go about it?

[westerman]

I don't use the software but from reading some help on it evidently in the text line above the peaks you will see the sequence. You can then add or delete bases.

I doubt that exporting the sequence and doing trimming outside the Sequence Scanner program (I use phred to do this work for the 3730 reads but undoubtedly there are many other programs) and then trying to re-import the sequence would work. It appears that the Sequence Scanner only works with trace files. So perhaps what you are really asking is 'how do I manipulate trace files?'