Hello everyone! I am using QIAEX II Gel Extraction Kit to purify PCR products (300 bp) from agarose gel. The final elution is in 20 ul of H2O. I use 5 ul of the eluate in a BigDye Terminator sequencing reaction and then I purify my product using a Membrane-based filter plate. The sequence I get is unfortunately very bad quality , with some spikes and a lot of noise. Do you think I should change seq purifiction method?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM