Hello everyone! I am using QIAEX II Gel Extraction Kit to purify PCR products (300 bp) from agarose gel. The final elution is in 20 ul of H2O. I use 5 ul of the eluate in a BigDye Terminator sequencing reaction and then I purify my product using a Membrane-based filter plate. The sequence I get is unfortunately very bad quality , with some spikes and a lot of noise. Do you think I should change seq purifiction method?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM