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Hi y'all, I left my complete sequencing reaction at room temp right outside the thermocycler by accident for about ~45 min b/c I was waiting for the lid to heat and forgot about it. It's the normal Big Dye terminator sequencing rxn, forward or reverse primer, big dye, water, sequencing buffer. I found my mistake and quickly put it into the PCR machine. DO you think it will be ok? I do. Of course BDTv3.1 is light sensitive but it was kinda in the shade, not under direct lights.. I hope 45 min isn't too long. Thanks!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
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