Hello all,
I have had several troubleshooting attempts at sequencing a low-diversity, small amplicon library on a NextSeq2000 using P1 50...
13 results in 0.0030 seconds.
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Small amplicon library sequencing
Last edited by CitizenSnips; 09-08-2025, 06:16 AM. -
Problems sequencing a low diversity library on NextSeq
Hi everyone,
We’ve done several attempts at sequencing a low diversity library on a NextSeq Mid Output v2.5 flow cell, following the... -
MiSeq Error in LanePump
Hi All,
We just ran a MiSeq 2x300bp amplicon sequencing run over the weekend and it has completed with decent specs but has shown an error... -
Trouble of First omni-ATAC-seq
Hello, I am a new user trying to figure out Omin-ATAC-seq. I did all the process according to paper Howard Y Chang (2017).
In qPCR amplification,...Last edited by PapayaWang; 03-09-2018, 12:36 PM. -
PolyA Enrichment Failed, Now What Do I Do?
Hi guys,
I haven't had this happen to me in a few years: I used Sera-Mag Oligo-dT beads with homemade buffers to enrich polyA RNA from... -
ATAC-seq plasmid contamination after transfection
HI all
I am trying to produce ATAC-seq libraries from transfected cells, but it seems a pain...
I have produced good libraries... -
Reason for POOR per tile sequence quality
We recently performed a sequencing run on the MiSeq sequencer using a V3 (600 cycle kit).
With regards to the per tile quality plot (obtained... -
RNA Purification Beads during First and Second Strand Synthesis
I'm using the Tru-seq Stranded mRNA Sample Preparation kit and I made the mistake of not removing the RNA from the RNA Purification Beads before starting... -
TapeStation 2200: multiple problems
Dear users,
this is my first post at SEQanswers. I work at a NGS core facility as scientific head, which involves a lot of troubleshooting.... -
Laboratory Supervisor at NY Genome Center
About the New York Genome Center
The New York Genome Center (NYGC) is an independent, non-profit organization that leverages the collaborative resources...Last edited by New York Genome Center; 06-13-2014, 01:09 PM. -
MiSeq Run, Problem with Index reads
I just ran a miseq run using NEBNext Ultra DNA prep, (the bio analyzer results looked fine prior to sequencing).
We sequenced 6 samples... -
Sequencing rxn left at room temp for ~45 min
Hi y'all, I left my complete sequencing reaction at room temp right outside the thermocycler by accident for about ~45 min b/c I was waiting for the lid... -
Long Range PCR problem...
Hi Guys,
So I'm trying to amplify whole mtDNA of a few species using long-range PCR. I've found a conserved region and designed long (30mer)...
