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  • mohd2b
    Junior Member
    • Nov 2014
    • 9
    #1

    3130 genetic analyzer raw data range

    hi

    Well for those who have used 3130 genetic analyzer, when you look in the raw data of a run , how do you interrupt the results?

    in my case , most of the time I got a raw data signal intensity below 100 and sometimes below 200!!. However, electropherogram of the run looks good with QV > 20 which can be further assembled and analyzed. Should I trust the results despite low signal intensity value of the raw data?

    Please share your thought about it

    thanks
    Tags: None
    • mohd2b
      Junior Member
      • Nov 2014
      • 9
      #2
      here are some image of my sequence
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