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  • sharones
    Junior Member
    • Dec 2015
    • 1
    #1

    Seq of PCR product with great seq but one peak very low intensity

    Hello,

    I have not encountered anything like this before so I'm hoping someone could shed some light. I have a beautiful sequence for a PCR product and have noticed that although the rest of the sequence has uniform intensity, I have one peak that is so drastically diminished in its intensity that the software calls it a deletion, however, upon closer inspection the peak is very very small. What could cause this? Thanks!
    Tags: None
    • MU Core
      Member
      • Apr 2008
      • 60
      #2
      Sharones, would you be able to post the image of the peak trace?

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