Strange migration in fragment analysis

[tangaraud]

Hi
Since few days we have strange migration on our ABI 3130.
We change the capilary and it was the ok for two days then the problem came back.
I have reads lots of troubleshooting guides but nothings comparable.....
Every consumable were changed but the problem still there.
Is anyone of you have a idea of this problem type?
In attachement an exemple of LIZ500 migration (capillary 2 and 3 presents strange migrations)
Thanks a lots for your help

T

[finswimmer]

Hello,
try changing the polymer, also refill the array with the new polymer. Check for bubbles in the fluid system. What about the room temperature? We see those problems more often, if the room temperature is to high or vary to much.

Hope that helps a bit.

fin swimmer

[tangaraud]

Hello Fin,

We have changed also the reagents. New lots of polymer, buffers, HiDi, LIZ500, but no amelioration.
The température is 24°c in the lab and is stable +/-0.5°c during the last week.
Thanks for your help.


T