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Hello all,
Newbie to the forum here, and also new to the 3130XL! My lab is upgrading from the ABI 310 and I have a few stupid questions:
-can we continue to use the same POP4 polymer?
-do the septa actually need to be removed when denaturing the plate as the manual recommends? If so, what do you use in place of the septa?
-with the ABI 310, we use our own size standard and FAM/TET/HEX/TAM dyes. From what I read, I think we can set up our own 4 dye spectral calibration to set up a new matrix for each capillary. Is this correct?
-any other tips to save in time and money would be appreciated! As you all know, these machines (and their reagents) are not cheap!
Thanks in advance for your help!
Newbie to the forum here, and also new to the 3130XL! My lab is upgrading from the ABI 310 and I have a few stupid questions:
-can we continue to use the same POP4 polymer?
-do the septa actually need to be removed when denaturing the plate as the manual recommends? If so, what do you use in place of the septa?
-with the ABI 310, we use our own size standard and FAM/TET/HEX/TAM dyes. From what I read, I think we can set up our own 4 dye spectral calibration to set up a new matrix for each capillary. Is this correct?
-any other tips to save in time and money would be appreciated! As you all know, these machines (and their reagents) are not cheap!
Thanks in advance for your help!