I am just now exploring the indel pipeline for SOLiD. Of course this has only recently been released. Unfortunately I am obtaining very few results. My understanding is that the pairing pipeline is suppose to produce a '*.pas' file that would contain possible indels. Then the indel pipeline takes this and produces the true indels. For some reason the pairing pipelines is creating very small '*.pas' files -- no more than 4 lines per chromosome and often less. Additionally the 'PAIR_INDEL_*.sh' command is running very quickly. No errors are showing up (e.g., out of disk space, etc.) and thus I am wondering what is happening. Any ideas would be appreciated.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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