Hi all,
Even if I am quite new to sequencing, my boss and I decided to initiate talks about next-gen sequencing in our university so that we could stay updated.
I am preparing a talk where I am briefly describing the three most known technologies (454, Illumina and SOLiD). While I think I got the principle of the two first approaches, I still have some questions about the SOLiD approach. On the web, I could find explanations going in every directions.
So... I decided it it would be easier to ask experts (hopefully, they have time to answer me).
Of course, if someone knows a very good website / paper explaining very clearly the SOLiD technologies, please give me the URL!
My questions :
a) At each step of the cycle, are the 16 possible oligonucleotides added or only four, then which one?
b) Which bases of the oligonucleotides are the "interesting" one? The first and the second or the fourth and the fifth?
I may have more questions ... but this is already a good start. Many many thanks to you all for your help.
Regards
Even if I am quite new to sequencing, my boss and I decided to initiate talks about next-gen sequencing in our university so that we could stay updated.
I am preparing a talk where I am briefly describing the three most known technologies (454, Illumina and SOLiD). While I think I got the principle of the two first approaches, I still have some questions about the SOLiD approach. On the web, I could find explanations going in every directions.
So... I decided it it would be easier to ask experts (hopefully, they have time to answer me).
Of course, if someone knows a very good website / paper explaining very clearly the SOLiD technologies, please give me the URL!
My questions :
a) At each step of the cycle, are the 16 possible oligonucleotides added or only four, then which one?
b) Which bases of the oligonucleotides are the "interesting" one? The first and the second or the fourth and the fifth?
I may have more questions ... but this is already a good start. Many many thanks to you all for your help.
Regards
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