Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Why are you using command line? You can import them in and start the assembly using the GUI interface.
-
Thank you for your quick answer.
I will try to use the graphical interface as it is aparently more freindly...
I tried to use the command line for the novo assembly that is called clc_novo_assemble and it seems like it only accepts fastq files as input while I have csfasta+qual files
I would have liked to know if someone uses command line clc de novo assembly with color space data ...
Leave a comment:
-
No, you don't need to convert to fastq if you're using the graphical CLC bio version 4.8+ or probably many earlier versions (I don t know what clc_novo_assmble is!).
In general, you definitely want to stay in colour space with Solid assemblies if at all possible.
Leave a comment:
-
how to use CLC bio software for assemly with SOLiD
Hello all,
I am completely new commer to NGS technology and SOLiD platform.
I want to do de novo assembly of SOLiD data with the CLC bio software
clc_novo_assmble. apprently this software accepts fastq files for the assembly while I have .csfasta+ qual files. do I need to convert the csfasta+qual to fasq first? do I need to translate it from color space to nt space before doing the assembly?
Thanks for your helpTags: None
Latest Articles
Collapse
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 11:49 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
Yesterday, 11:49 AM
|
||
Started by seqadmin, 04-24-2024, 08:47 AM
|
0 responses
16 views
0 likes
|
Last Post
by seqadmin
04-24-2024, 08:47 AM
|
||
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
61 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
60 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
Leave a comment: