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Hello, both tubes (enriched, unenriched) are filled with transparent fluid, seems like watter, without anything inside. Considering the filter, it seemed to be empty, no magn.beads within. I tried to reuse it another day and some of mag. beads remaining in the Enricher Input tube easily passed it. On the contrary, the new filter worked properly, magn. beads taken from the Enricher Input Tube remained within. So what next? Is the filter supposed to be the key problem? Thanks for your answer. -
Your beads really shouldn't end up there even if the emPCR is failing. What do you see in your enriched and un-enriched tubes? is there anything at all or are they completely empty? also, how does your filter look?Leave a comment:
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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