Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Poor Bead assesment report and scattered satays for SOLiD 5500xl run

    Hi all,

    In our lab we have installed the SOLiD 5500xl machine recently. So for a test run (25bp fragment run) we had used one of the old libraries(post e-PCR and 3'modification) that we had already prepared (this library was already run on another SOLiD 5500xl machine previously and the Bead assessment report that we had got was pretty good.). But for the current trial run the bead assessment report that we have got is poor with a N2S ratio of 65% and %on axis is only 2%.
    The satays obtained for the ligation steps were also fully scattered initially and then used to concentrate at the centre (Refer the attached images for the satays)

    After loading the beads on the flowchip,the flowchip was stored at 4C for upto a week due to some problem with the instrument. After the problem was rectified we had started the run.

    Has anybody faced a similar problem?? Would storing the flowchip with beads could have caused this problem?? Or could it be some other reason? Kindly help.. Thank u ..

    Jaya
    Attached Files

  • #2
    Hi Jaya, yes we have had the same problem. If you can't run the instrument straight away it's much better to store the beads before the 3' modification. If you do load the chip then get an instrument problem all you can do is get that run started as soon as possible.

    JPC

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Multiomics Techniques Advancing Disease Research
      by seqadmin


      New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

      A major leap in the field has
      ...
      02-08-2024, 06:33 AM
    • seqadmin
      The 3D Genome: New Technologies and Emerging Insights
      by seqadmin


      The study of three-dimensional (3D) genomics explores the spatial structure of genomes and their role in processes like gene expression and DNA replication. By employing innovative technologies, researchers can study these arrangements to discover their role in various biological processes. Scientists continue to find new ways in which the organization of DNA is involved in processes like development1 and disease2.

      Basic Organization and Structure
      Understanding...
      01-22-2024, 03:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 08:57 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-14-2024, 09:19 AM
    0 responses
    43 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-12-2024, 03:37 PM
    0 responses
    408 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-09-2024, 03:36 PM
    0 responses
    648 views
    0 likes
    Last Post seqadmin  
    Working...
    X