Hi all,
In our lab we have installed the SOLiD 5500xl machine recently. So for a test run (25bp fragment run) we had used one of the old libraries(post e-PCR and 3'modification) that we had already prepared (this library was already run on another SOLiD 5500xl machine previously and the Bead assessment report that we had got was pretty good.). But for the current trial run the bead assessment report that we have got is poor with a N2S ratio of 65% and %on axis is only 2%.
The satays obtained for the ligation steps were also fully scattered initially and then used to concentrate at the centre (Refer the attached images for the satays)
After loading the beads on the flowchip,the flowchip was stored at 4C for upto a week due to some problem with the instrument. After the problem was rectified we had started the run.
Has anybody faced a similar problem?? Would storing the flowchip with beads could have caused this problem?? Or could it be some other reason? Kindly help.. Thank u ..
Jaya
In our lab we have installed the SOLiD 5500xl machine recently. So for a test run (25bp fragment run) we had used one of the old libraries(post e-PCR and 3'modification) that we had already prepared (this library was already run on another SOLiD 5500xl machine previously and the Bead assessment report that we had got was pretty good.). But for the current trial run the bead assessment report that we have got is poor with a N2S ratio of 65% and %on axis is only 2%.
The satays obtained for the ligation steps were also fully scattered initially and then used to concentrate at the centre (Refer the attached images for the satays)
After loading the beads on the flowchip,the flowchip was stored at 4C for upto a week due to some problem with the instrument. After the problem was rectified we had started the run.
Has anybody faced a similar problem?? Would storing the flowchip with beads could have caused this problem?? Or could it be some other reason? Kindly help.. Thank u ..
Jaya
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