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RNA has to be reverse transcribed (turned into DNA) before it can be sequenced, even if there was RNA in your sample it would not be present in your data -
DNA sequencing and RNA contamination
There might have been some RNA present in gDNA sample that was sequenced with SOLID. How can one distinguish between mutations found in DNA and those in RNA contaminating the sample?
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM
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