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SOLiD5500 (total) meltdown



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  • SOLiD5500 (total) meltdown


    we are experiencing problems with our 2 SOLiD5500 machines since end of july. It is getting extremely frustrating because one run the reverse quality is crap (<40% good best), another one the forward is crap (<20% good best)and the next one the barcode is crap (<60% good best).

    Is there anyone else experiencing problems with their machines when comparing results to previous ones?

    Sidenote: we do see that lane 1 is routinely better than lane 6, although lifetech assured us this was impossible.

  • #2
    Are you certain the library titration was correct? Best beads % can be low from too much DNA in bead prep.


    • #3
      Yes I am sure the samples are good, we did run them previously without problems. The noise/signal and on axis are all ok.

      The problem does not appear to be the machine so now we are testing another EZbead station and another lot-nr chemistry.


      • #4
        I give up.


        • #5
          that does not look like solid data. shouldn't there be cycles of quality?


          • #6
            You mean like this?

            Last bar of the first ligation cycle is base 71
            Last bar of the second ligation cycle is base 72



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