Header Leaderboard Ad

Collapse

SOLiD5500 (total) meltdown

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SOLiD5500 (total) meltdown

    Hello,

    we are experiencing problems with our 2 SOLiD5500 machines since end of july. It is getting extremely frustrating because one run the reverse quality is crap (<40% good best), another one the forward is crap (<20% good best)and the next one the barcode is crap (<60% good best).

    Is there anyone else experiencing problems with their machines when comparing results to previous ones?

    Sidenote: we do see that lane 1 is routinely better than lane 6, although lifetech assured us this was impossible.

  • #2
    Are you certain the library titration was correct? Best beads % can be low from too much DNA in bead prep.

    Comment


    • #3
      Yes I am sure the samples are good, we did run them previously without problems. The noise/signal and on axis are all ok.

      The problem does not appear to be the machine so now we are testing another EZbead station and another lot-nr chemistry.

      Comment


      • #4
        I give up.

        Comment


        • #5
          that does not look like solid data. shouldn't there be cycles of quality?

          Comment


          • #6
            You mean like this?



            Last bar of the first ligation cycle is base 71
            Last bar of the second ligation cycle is base 72

            etc.

            Comment

            Latest Articles

            Collapse

            • seqadmin
              A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
              by seqadmin


              ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

              01-24-2023, 01:19 PM
            • seqadmin
              Introduction to Single-Cell Sequencing
              by seqadmin
              Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

              The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
              ...
              01-09-2023, 03:10 PM

            ad_right_rmr

            Collapse
            Working...
            X