I don't yet have first-hand experience with the Illumina workflow. But I would suspect that in cases where you are doing "quad" or "oct" runs on a SOLiD flow cell, bead prep is substantially more work than cluster generation on the Illumina. The process VanessaS describes above would be "x4" for a quad run.
The reagent costs for doing anything other than a full flow cell (E80) run on the EZBead make it not cost-effective compared to the manual method. Or, at least, of questionable cost-effectiveness.
VanessaS, do you do quad runs on your SOLiDs? If so, do you use the EZBead for them as well?
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Phillip
The reagent costs for doing anything other than a full flow cell (E80) run on the EZBead make it not cost-effective compared to the manual method. Or, at least, of questionable cost-effectiveness.
VanessaS, do you do quad runs on your SOLiDs? If so, do you use the EZBead for them as well?
--
Phillip
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