Epicentre's ScriptMiner Kit has been improved with a streamlined protocol that requires less hands-on time. The ScriptMiner Kit produces nonbarcoded or barcoded Illumina sequencing libraries from small RNA, including miRNA. The kit contains Tobacco Acid Pyrophosphatase (TAP), enabling capture of small 5´-capped RNA and small 5´-triphosphorylated RNA. A unique enzymatic procedure greatly reduces adaptor-dimer formation in the sequencing library.
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Hi,
thanks for the post. I have 2 questions about the new protocol: What is the optimum temperature for the Scriptminer 3'-RNA and the 5'-RNA ligase? I have to do the incubation in the thermocycler as our lab is too cold.
The PCR amplification step states to use 40-45 uL di-tagged cDNA - however, we have only 40uL cDNA in total after the cDNA synthesis. Is that a typo?
thank you,
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