Hi all,
I am new in RRBS. I want to use the gel-free protocol descripted in the article of Boyle et al. Genome biology 2012, 13. I want...
40 results in 0.0034 seconds.
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RRBS acceptable coverage
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Exome multiplexing
Hi,
have anyone tried to multiplex samples and targeted for higher coverage in samples vs. controls. Eg. as in the truseq exome enrichment... -
Multiplexing Transcription factor Chip-seq
Hi all,
We will be soon starting to do libraries from Transcription factor (TF) Chip-seq, for a genome of ~ 330 Mb.
We want... -
Multiplex scheme for Hiseq run
Hi,
I have an issue I need to resolve regarding multiplexing libraries for a Hiseq run.
There are 96 libraries, I am making... -
Amplicon Sequencing with Ion Torrent
Hi, I've been reading threads in this forum for months now and have found a lot of useful information, and seen many questions answered and figured id... -
Barcodes & biases
Hi there,
Has anyone observed barcode-specific biases in his/her NGS samples?
I am aware of the miRNA-seq papers on that... -
MiSeq Bacterial Genome Multiplexing
Can I multiplex bacterial genomes on MiSeq? If so, how many ? Thank you! -
Minimum dsDNA detection by NGS machine
Hello,
I am new here and to NGS. I prepared some multiplex DNA libraries with Illumina TrueSeq Kit. I submitted each sample/index at 10nM,... -
TruSeq RNA-How many samples to pool?
Hey,
I would like to pool only 4 or 5 TruSeq RNA libraries and i am wondering which indexes to used? Previously, Illumina provided some... -
multiplex few variants in hundreds of samples
Dear all,
I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing... -
Index read problem
Hi everyone, I'm a new user of Hiseq, this is a first time that I'm running a multiplex run and I have some concerns about Index read. Until the index... -
HiSeq multiplexing for strand specific RNA library prep
Greetings, fellow scientists!
I am a grad student and my first task in lab is to pioneer a gene expression experiment to be run on the... -
Postdoc at Trent University/Canada
Hi there,
ok, a short introduction:
My name is Conny and I'm working as a postdoc at Trent University in Peterborough/Canada.... -
Demultiplexing and CASAVA 1.7
Hello,
I am looking for young bioinformatics. I need tools to perform demultiplexing
tool (script) to sequences from the GAIIx before... -
Illumina Pair end primers and adaptors for multiplexing
I would really appreciate any help with being able to decipher the different primers and adaptors in the illumina library preparation kits.
...
