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  • mikev
    Junior Member
    • Feb 2013
    • 1
    #1

    RRBS acceptable coverage

    Hi all,

    I am new in RRBS. I want to use the gel-free protocol descripted in the article of Boyle et al. Genome biology 2012, 13. I want to use RRBS for a population based studie. What is an acceptable coverage per CpG for this type of studie?
    I ask this to determine the maximal acceptable number of samples per lane. I want to run on a hiseq 2000.
    Tags: coverage depth, epigenetics, methylation, multiplexing, rrbs
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