Hello to everyone. I am new in this forum.
I am working on a library for reduced representation bisulfite sequencing (RRBS). The protocol...
34 results in 0.0032 seconds.
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RRDS adapter mismatch design
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Best way to trim MiSeq - NEBNext Multiplex Oligos for Illumina Library
I have a 51 single-end reads generated with MiSeq using NEBNext Multiplex Oligos for Illumina.
The sample sheet looks like this:
...Last edited by foolishbrat; 01-26-2014, 10:33 PM. -
MiSeq Index Primer Sequence
Hi all,
I am looking for the exact sequence for the default MiSeq Index Primer. Can anyone help me to check if this is the correct one?... -
NEWBIE question: does the 5' adapter mean forward primer?
As in the title.
3' adapter == reverse primer
5' adapter == foward primer
Is that right? Thanks!
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Any idea on primer trimming tool?
I was looking at some sequence data generated by Sanger Sequencer ABI 3730 xl. Is there any recommended tool to do the primer trimming?
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function of a linker sequence within a primer
Hi guys,
I will ask you a probably easy question:
What is the general function of the linker sequence in primers in and in particular... -
Softclipping of primers for Fluidigm / Raindance PCR based methods
When performing target enrichment of large panels of genes using a PCR based method such as Fluidigm or Raindance, primers of varying lengths are used... -
multiplex primer design software (is there any?)
Hi guys,
I'm wondering if there is any software for multiplex PCR primer designs.
I know about Primer3 and that a lot... -
Miseq illumina primer
Hi,
I am new here and also in sequencing area. I am not sure if I can ask such a basic question here or not. Actually, I'd like to sequence... -
Tagmentase Adapters
I have a general question about the tagmentase reaction. Has anyone experimented with using single-adapter tagmentase complexes in pairs? For e.g. instead... -
TruSeq DNA - need index/barcode-specific primers
Hi there,
I have to pool libraries from different sources and labs. One of our collaborator don't have the index information of his library anymore.... -
Sureselect kits
Hello,
Does anyone know the difference between the SureSelect Primer in the Sureselect library prep kit ILM and the InPE primer 1.0 in the Sureselect... -
barcodes from recent papers...better?
Hello,
I've been wondering what barcodes to use for some 454 amplicon sequencing: A couple of new publications confidently suggest that their... -
Sorting fastq by primers, then searching by sequence (with mismatches)
Hi all, after my near-immediate success solving my last problem on here I thought I'd ask about my other major struggles!
I'm currently... -
fastqc - overrepresented sequences
I have run a FASTQC analysis and found out that there are hundreds of over-represented sequences in my datasets. Some of these, are Illumina PCR primers,...
