NEWBIE question: does the 5' adapter mean forward primer?

[arkilis]

As in the title.

3' adapter == reverse primer
5' adapter == foward primer

Is that right? Thanks!

[Kennels]

No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.

[arkilis]

No, the adapters are not the primers.
5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.

thx~~ it is much clear to me now