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Hi,
I am new here and also in sequencing area. I am not sure if I can ask such a basic question here or not. Actually, I'd like to sequence my 16S rRNA data for 3 different regions (v1-v3, v3-v5 and v6-v9). I have to design three different primer for sequencing with Miseq illumina system. I have read (Cporaso 2010 and 2012) papers but I have still problem with designing primers:
1- I don't know where I can find the Miseq illumina adaptor.
2- If it is some sequence constant for all regions or should be different.
3- How can I design the pad part.
4- The linker part.
5- The barcode
6- And also 12-base error-correctong Golay.
Could anyone explain them for me or any basic and advanced literatures I can learn from?!
Thanks,
Rozita
I am new here and also in sequencing area. I am not sure if I can ask such a basic question here or not. Actually, I'd like to sequence my 16S rRNA data for 3 different regions (v1-v3, v3-v5 and v6-v9). I have to design three different primer for sequencing with Miseq illumina system. I have read (Cporaso 2010 and 2012) papers but I have still problem with designing primers:
1- I don't know where I can find the Miseq illumina adaptor.
2- If it is some sequence constant for all regions or should be different.
3- How can I design the pad part.
4- The linker part.
5- The barcode
6- And also 12-base error-correctong Golay.
Could anyone explain them for me or any basic and advanced literatures I can learn from?!
Thanks,
Rozita
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