Dear SEQanswers friends,
We have taken an earthworm genome to the final round of the 2016 SMRT Sequencing Grant Program “Explore your...
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Untangling The Volcanic Earthworm Genome
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SMRTbell Synthesis and Sequence
- Are SMRTbells made by synthesis?
- If so, how are they purified (desalting, FPLC, etc...)?
- What is the SMRTbell sequence (not barcoded... -
20K Internal Control
I'm aware of the 2K PacBio Internal Control -- but smaller inserts function to control different things and tend to represent the best-case scenario (loading... -
Developing microsatellites from pacbio reads
Disclaimer: everything I know about Pacbio has been learned over the process of the last few days. I'm basically the closest thing to a bioinformatician... -
Error correction of PACbio using Illumina? When and how? no Ref. Genome
Hi all,
We've sequenced the transcriptom of a species (genome is about 3GB), but NO reference genome is available, using PACbio and Illumina.... -
Is this a valid [CircularConsensus] Result Report?
Hi all,
I'm new to pacbio analysis. It's my first time to run SMRT analysis to get read of insert. I used the following command:
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Uploading PacBio raw data to ENA SRA
Surprisingly I was not able to find anything on Google about the details of uploading raw PacBio sequence reads to the European Nucleotide Archive (ENA),... -
Basic Coverage Calculation Questions
For the sequencing technologies ABI 3730, 454, Illumina, and PacBio, what would the number of reads be to ensure 99.995% coverage of the human genome... -
Help: de novo assembly using SMRT portal and hgap.3
Problem: Extremely new to assembly and attempting to execute de novo assembly using hgap.3 in SMRT portal using 9 SMRTcells. lambda example data provided... -
Finding T-DNA insertion events in complex genome
Hi, I need to design a sequencing experiment to locate about a dozen insertion events of a ~10kbp transgene of known sequence in a very complex plant...Last edited by flef; 02-24-2016, 04:57 PM. -
how to do methylation analysis using pacbio data
I want to do methylation analysis based on pacbio .bax.h5 data. I already mapped the .h5 data to the reference genome and got .cmp.h5/.sam results. ... -
ROI_Mapping: difference between Mapped ROI total and Mapped ROI total (bp)
Hello,
I am new to PacBio and SMRT sequencing. Today I submit the job through SMRT portal: ROI_mapping and received the analysis back. However,... -
How do I get the RQ= information in the header of the PB fastq ou fasta files
Hi,
I've extracted fastq and fasta from bas.h5 files using bash5tools.py, but the output file did not contain the RQ= information in the... -
How can I calcualte the sequence identity and coverage form GMAP output bam file?
Now I have got a bam file from gmap alignment with data from pacbio isoseq result. Now I want to get the statistic information about the coverage and...Last edited by huan; 01-20-2016, 12:37 AM. -
Correcting PacBio with a hybrid approximation
Hello everyone,
I have PacBio data and I would like to correct them with Illumina HiSeq short reads. I test several programs and finally...
