SMRTbell Synthesis and Sequence

[davisc]

- Are SMRTbells made by synthesis?
- If so, how are they purified (desalting, FPLC, etc...)?
- What is the SMRTbell sequence (not barcoded and barcoded)?
- What is the sequence of the primer binding site?
- What is the concentration of the SMARTbells used in the ligation reaction?

Thanks.

[rhall]

Some of this information is public, some isn't. For adapter sequences, look in any *.metadata.xml file from a sequencing run. The adapter forms a hairpin, so any barcode sequence just needs to be added to the ends in the correct orientations. Adapter concentrations can be found in the template prep protocols http://www.pacb.com/support/document..._sort=preserve