Basic Coverage Calculation Questions

as29059

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Basic Coverage Calculation Questions

03-08-2016, 11:33 AM

For the sequencing technologies ABI 3730, 454, Illumina, and PacBio, what would the number of reads be to ensure 99.995% coverage of the human genome under this model.
From there for each technology, how would I also estimate the total cost of this sequencing?

Please help. what i have so far is below:

L= read lengths
ABI = 800,454: 700
Illumina= 300
PacBio= 500
N= # of bases sequenced = ?
C= 10
e=2.718
= c= LN/G
G= 3.2
10=?/3.2b
3.2bx10=32 reads necessary in order to ensure 99.9995% coverage of human genome.

Thanks so much in advance!
Tags: abi, illumina, pacbio
ECO

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Join Date: Oct 2007

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#2

03-08-2016, 12:23 PM

Sounds like someone's Lander-Waterman homework?
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ECO

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Posts: 1360
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#3

03-08-2016, 12:23 PM

Oh and you crossposted it a few times...tsk tsk
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