For the sequencing technologies ABI 3730, 454, Illumina, and PacBio, what would the number of reads be to ensure 99.995% coverage of the human genome under this model.
From there for each technology, how would I also estimate the total cost of this sequencing?
Please help. what i have so far is below:
L= read lengths
ABI = 800,454: 700
Illumina= 300
PacBio= 500
N= # of bases sequenced = ?
C= 10
e=2.718
= c= LN/G
G= 3.2
10=?/3.2b
3.2bx10=32 reads necessary in order to ensure 99.9995% coverage of human genome.
Thanks so much in advance!
From there for each technology, how would I also estimate the total cost of this sequencing?
Please help. what i have so far is below:
L= read lengths
ABI = 800,454: 700
Illumina= 300
PacBio= 500
N= # of bases sequenced = ?
C= 10
e=2.718
= c= LN/G
G= 3.2
10=?/3.2b
3.2bx10=32 reads necessary in order to ensure 99.9995% coverage of human genome.
Thanks so much in advance!
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