Hi all, I am quite new to Illumina amplicons library preparation. I am having issue regarding adaptors ligation.
I figure out the problem...
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Illumina library preparation - adaptor ligation
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NGS analysis, questions to ask ...
Before taking up NGS data for analysis interesting questions raised early on can pretty much foster a successful analysis through. This ensures no communication... -
Roche 454 RL adapters.. help!!!
Hi,
We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We... -
ChIP-Seq Library Perp
Hi,
I have been trying to make a ChIP-Seq library using TruSeq Adapters. Even though the DNA profile looks good on the bioanalyzer, I'm... -
Expected Library size after adaptors
If we get a pre adaptor, size selected library with an average size of 170 bp then after the adaptors (which are 41 and 53 bp) I would expect to see 170+41+53=264bp... -
Illumina Pair end primers and adaptors for multiplexing
I would really appreciate any help with being able to decipher the different primers and adaptors in the illumina library preparation kits.
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Adapter sequences
I'm looking for the sequences of both the 454 FLX and the Titanium library adapters. I'd also be interested to know the adapter sequences of any of the...
