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  • BioSlayer
    Member
    • Feb 2010
    • 26

    NGS analysis, questions to ask ...

    Before taking up NGS data for analysis interesting questions raised early on can pretty much foster a successful analysis through. This ensures no communication breakdowns take place between scientist on both sides of the NGS story, the sequencing facility and the computational facility.

    As a bioinformatician it is interesting to me to have information on the type of experiment (DNA seq, RNA seq, assembly...etc), data (single end, paired end), platforms and throughput, depth and coverage of reads, samples/patients naming conventions, encoding, replicates, study design (whole genome, whole exon..etc), adaptor files if any. And foremost; on how far my capacity (load/experience/enthusiasm) can lead me.

    My short list maybe missing more interesting question so what are your expectations before accepting data for analysis depending on the type of experiment ?

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  • SEQadmin2
    Cancer Drug Resistance: The Lingering Barrier to Rising Survival
    by SEQadmin2



    Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

    There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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